[Effects of Shenqi compound recipe on cell cycle and apoptosis of ascites inducible hepatocarcinoma cell line H22].

AIM To observe the effects of Shenqi compound recipe (SQ) on cell cycle and apoptosis of hepatocarcinoma cell line H22 in-vitro and in-vivo. METHODS BALB/c mice bearing tumors were perfused with SQ for 10 days running. And then cytotoxic activity of mouse NK cells and macrophages and variation of cell cycle of H22 cells were observed. Inhibition of H22 cell proliferation and induction of the their apoptosis by SQ were analyzed by flow cytometry. RESULTS The tumor-suppre ssive rate of SQ was 65.68%. SQ could make H22 cells arrested at the S phase and induce their apoptosis. CONCLUSION SQ has significant anti-tumor effect both in-vitro and in-vivo. Its mechanism may be related to blocking of cell cycle and inducing apoptosis of H22 cells.